Production of high titrehelper-freerecombinant retroviral vectors by lipofection
نویسندگان
چکیده
منابع مشابه
Production of high titre helper-free recombinant retroviral vectors by lipofection.
High titre stocks of replication-defective retroviral vectors are generally prepared by stable introduction of cloned proviraJ DNA into packaging cell lines that supply in-trans all the proteins necessary for viral assembly (1). However, different vectors are packaged with different efficiencies and the expression of transgenes and activity of heterologous internal promoter elements may vary wi...
متن کاملA transient three-plasmid expression system for the production of high titer retroviral vectors.
We have constructed a series of MLV-based retroviral vectors and packaging components expressed from the CMV promoter and carried on plasmids containing SV40 origins of replication. These two features greatly enhanced retroviral gene expression when introduced into cell lines carrying the SV40 large T antigen. The two packaging components, gag-pol and env, were placed on separate plasmids to re...
متن کاملCharacterization of recombinant plasminogen activator production by primate endothelial cells transduced with retroviral vectors.
Retroviral vector-mediated expression of plasminogen activators (PAs) from endothelial cells (ECs) has been proposed as a potential therapeutic approach for intravascular thrombosis. To define the potential for gene transfer to increase fibrinolytic activity in a primate system, baboon ECs were transduced with retroviral vectors expressing wild-type and glycosylphosphatidylinositol-anchored uro...
متن کاملCharacterization of Recombinant Plasminogen Activator Production by Primate Endothelial Cells Transduced With Retroviral Vectors
Retroviral vector-mediated expression of plasminogen activators (PAS) from endothelial cells (ECs) has been proposed as a potential therapeutic approach for intravascular thrombosis. To define the potential for gene transfer to increase fibrinolytic activity in a primate system, baboon ECs were transduced with retroviral vectors expressing wild-type and glycosylphorphatidylinositol-anchored uro...
متن کاملTransduction of human embryonic stem cells by ecotropic retroviral vectors
The steadily increasing availability of human embryonic stem (hES) cell lines has created strong interest in applying available tools for gene transfer in murine cells to human systems. Here we present a method for the transduction of hES cells with ecotropic retroviral vectors. hES cells were transiently transfected with a construct carrying the murine retrovirus receptor mCAT1. Subsequently, ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1994
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/22.6.1117